A guide to SEM analysis procedures
A guide to SEM analysis procedures
Jan 5, 2022
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Budding yeasts were observed using an FE-SEM equipped with a cryo system. The yeasts were dropped directly onto a sample stub, rapidly frozen with slush nitrogen without prior fixation or dehydration, then cleaved and coated with 2 nm of Pt in the preparation chamber of the cryo system. Invagination structure unique to yeast cell membranes were observed in the exposed protoplasmic fracture (PF) face. In the enlarged view, particles with a diameter of approximately 15 nm were recognized and some
0:53
Budding yeasts were observed using an FE-SEM equipped with a cryo system. The yeasts were dropped directly onto a sample stub, rapidly frozen with slush nitrogen without prior fixation or dehydration, then cleaved and coated with 2 nm of Pt in the preparation chamber of the cryo system. Invagination structure unique to yeast cell membranes were observed in the exposed protoplasmic fracture (PF) face. In the enlarged view, particles with a diameter of approximately 15 nm were recognized and some
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